FOXL2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70013
![Flow Cytometry: FOXL2 Antibody - BSA Free [NBP2-70013]](https://resources.bio-techne.com/images/products/FOXL2-Antibody-Flow-Cytometry-NBP2-70013-img0005.jpg "Flow Cytometry: FOXL2 Antibody - BSA Free [NBP2-70013]")
![Flow Cytometry: FOXL2 Antibody - BSA Free [NBP2-70013]](https://resources.bio-techne.com/images/products/FOXL2-Antibody-Flow-Cytometry-NBP2-70013-img0002.jpg "Flow Cytometry: FOXL2 Antibody - BSA Free [NBP2-70013]")
![Immunohistochemistry-Paraffin: FOXL2 Antibody - BSA Free [NBP2-70013]](https://resources.bio-techne.com/images/products/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-70013-img0003.jpg "Immunohistochemistry-Paraffin: FOXL2 Antibody - BSA Free [NBP2-70013]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to a C-terminal portion of the human FOXL2 protein (between amino acids 350-376) [UniProt P58012]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FOXL2 Antibody - BSA Free
Immunohistochemistry-Paraffin: FOXL2 Antibody - BSA Free [NBP2-70013]
Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-70013] - Analysis of a FFPE tissue section of the human ovary using 1:200 dilution of FOXL2 antibody (NBP2-70013). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity.![Western Blot: FOXL2 AntibodyBSA Free [NBP2-70013]](https://resources.bio-techne.com/images/products/FOXL2-Antibody-Western-Blot-NBP2-70013-img0004.jpg "Western Blot: FOXL2 AntibodyBSA Free [NBP2-70013]")
Western Blot: FOXL2 AntibodyBSA Free [NBP2-70013]
Western Blot: FOXL2 Antibody [NBP2-70013] - Total protein from K562 and human testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-FOXL2 in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - BSA Free [NBP2-70013]](https://resources.bio-techne.com/images/products/FOXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-70013-img0006.jpg "Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - BSA Free [NBP2-70013]")
Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - BSA Free [NBP2-70013]
Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NBP2-70013] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-FOXL2 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Applications for FOXL2 Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
2.5 ug/mL
Immunocytochemistry/ Immunofluorescence
2-5 ug/mL
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
2 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: FOXL2
Long Name
Forkhead Box L2
Alternate Names
BPES, BPES1, PFRK, PINTO, POF3
Gene Symbol
FOXL2
Additional FOXL2 Products
Product Documents for FOXL2 Antibody - BSA Free
Product Specific Notices for FOXL2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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