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FOXL2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03941

Novus Biologicals, part of Bio-Techne
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NBP3-03941-100ul
NBP3-03941-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-376 of human FOXL2 (NP_075555.1). HAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXL2 Antibody - Azide and BSA Free

Western Blot: FOXL2 AntibodyAzide and BSA Free [NBP3-03941]

Western Blot: FOXL2 AntibodyAzide and BSA Free [NBP3-03941]

Western Blot: FOXL2 Antibody [NBP3-03941] - Western blot analysis of extracts of various cell lines, using (NBP3-03941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
FOXL2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - Azide and BSA Free [NBP3-03941] -

Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - Azide and BSA Free [NBP3-03941] - Immunofluorescence analysis of mouse ovary using FOXL2 Rabbit pAb (A16244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
FOXL2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - Azide and BSA Free [NBP3-03941] -

Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody - Azide and BSA Free [NBP3-03941] - Immunofluorescence analysis of rat ovary using FOXL2 Rabbit pAb (A16244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for FOXL2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXL2

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Long Name

Forkhead Box L2

Alternate Names

BPES, BPES1, PFRK, PINTO, POF3

Gene Symbol

FOXL2

Additional FOXL2 Products

Product Documents for FOXL2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FOXL2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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