Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FoxM1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 713 and 763 of human forkhead box M1 using the numbering given in entry NP_973731.1 (GeneID 2305).

Localization

Nuclear

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FoxM1 Antibody

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660] - Detection of Human FOXM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXM1 antibody NB100-74660 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74660 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. FoxM1 was successfully immunoprecipitated by another rabbit anti-FoxM1 antibodies and NB100-74660, which recognize upstream epitopes.

Applications for FoxM1 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FoxM1

FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. FOXM1 is ubiquitously expressed in proliferating cells but is absent in differentiated cells. FOXM1 overexpression has been detected in a variety of tumor types including breast, lung, prostate, and liver cancer, and appears to be involved in tumorigenesis-associated processes such as angiogenesis and metastasis. In light of these findings, FOXM1 has recently become an attractive target for anti-cancer therapy.

Long Name

Forkhead Box M1

Alternate Names

FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP-2, PIG29, Trident

Entrez Gene IDs

2305 (Human)

Gene Symbol

FOXM1

UniProt

Q08050

Additional FoxM1 Products

Product Documents for FoxM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FoxM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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