Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 83N7F8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all FoxO1/FKHR Antibodies »

Product Specifications

Immunogen

FOXO1A

Localization

Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free

Western Blot: FoxO1/FKHR Antibody (83N7F8)Azide and BSA Free [NBP2-80741]

Western Blot: FoxO1/FKHR Antibody (83N7F8)Azide and BSA Free [NBP2-80741]

Western Blot: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741]

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741]

Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741]

Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as
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Applications for FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.1-2 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

0.5-2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

The FOX1A transcription factor is a member of forkhead family of transcription factors. It is the main target of insulin signaling. When insulin is present, its activity is suppressed. It regulates metabolic homeostasis in response to oxidative stress. It has several other functions: main regulator of redox balance, control osteoblast numbers, promotes gluconeogenesis (hepatocytes), cell death regulation, promote neural cell death, regulation of adipogenic genes, regulate transcriptional activity of GADD45A, and repair nitric oxide-damaged DNA (beta-cells).

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Gene Symbol

FOXO1

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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