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FOXO3 [p Ser253] Antibody (SR2309)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21531

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21531-100ul
NBP3-21531-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2309

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for FOXO3 [p Ser253] Antibody (SR2309)

Immunogen

A synthesized peptide derived from human phospho-FOXO3 [Ser253] (Uniprot #: O43524)

Modification

p Ser 253

Specificity

Detects endogenous levels of total phospho-FOXO3 [Ser253]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXO3 [p Ser253] Antibody (SR2309)

Immunohistochemistry: FOXO3 [p Ser 253] Antibody (SR2309) [NBP3-21531] -

Immunohistochemistry: FOXO3 [p Ser 253] Antibody (SR2309) [NBP3-21531] - Immunohistochemical analysis of paraffin-embedded human uterus cancer, using NBP3-21531.

Western Blot: FOXO3 [p Ser 253] Antibody (SR2309) [NBP3-21531] -

Western Blot: FOXO3 [p Ser 253] Antibody (SR2309) [NBP3-21531] - Western blot analysis using NBP3-21531 in MCF-7 cell lysate treated with IGF.

Applications for FOXO3 [p Ser253] Antibody (SR2309)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 [p Ser253] Antibody (SR2309)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FOXO3 [p Ser253] Antibody (SR2309)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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