FOXO3 [p Ser425] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16945

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FoxO3 Antibodies »

Product Summary for FOXO3 [p Ser425] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around S425 of human FOXO3 (NP_001446.1). LGSPT

Modification

p Ser 425

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOXO3 [p Ser425] Antibody - Azide and BSA Free

Western Blot: FOXO3 [p Ser425] AntibodyAzide and BSA Free [NBP3-16945]

Western Blot: FOXO3 [p Ser425] AntibodyAzide and BSA Free [NBP3-16945]

Western Blot: FOXO3 [p Ser425] Antibody [NBP3-16945] - Western blot analysis of extracts of K-562 cells, using FOXO3 antibody (NBP3-16945) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]

Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]

Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded mouse heart using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]

Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]

Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded rat kidney using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for FOXO3 [p Ser425] Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 [p Ser425] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FOXO3 [p Ser425] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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