FoxP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-243

Novus Biologicals, part of Bio-Techne
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NB600-243
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRSQRPNKCSNP corresponding to C-Terminus according to Uniprot Mouse NP_473380.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FoxP3 Antibody

FoxP3 Antibody

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] -

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: FoxP3 Antibody [NB600-243]

Flow Cytometry: FoxP3 Antibody [NB600-243]

Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.

Applications for FoxP3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:4000
Application Notes
IHC-P: Mouse thymus shows nuclear staining in some patches of thymocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxP3

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse); 317382 (Rat)

Gene Symbol

FOXP3

UniProt

NP_473380.1 (Mouse)

Additional FoxP3 Products

Product Documents for FoxP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FoxP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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