FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71351

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI12F9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FRA-1 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human FOSL1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free

Western Blot: FRA-1/FOSL1 Antibody (OTI12F9)Azide and BSA Free [NBP2-71351]

Western Blot: FRA-1/FOSL1 Antibody (OTI12F9)Azide and BSA Free [NBP2-71351]

Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.
Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351]

Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351]

Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.
Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351]

Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351]

Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.
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Applications for FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FRA-1

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

Long Name

Fos-related antigen 1

Alternate Names

FOSL1, FRA1

Gene Symbol

FOSL1

Additional FRA-1 Products

Product Documents for FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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