Frataxin Antibody (3G9)
Novus Biologicals, part of Bio-Techne | Catalog # H00002395-M03
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3G9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
FXN (AAH48097.1, 91 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDL
Specificity
FXN (3G9)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Frataxin Antibody (3G9)
![Western Blot: Frataxin Antibody (3G9) [H00002395-M03]](https://resources.bio-techne.com/images/products/Frataxin-Antibody-3G9-Western-Blot-H00002395-M03-img0003.jpg "Western Blot: Frataxin Antibody (3G9) [H00002395-M03]")
Western Blot: Frataxin Antibody (3G9) [H00002395-M03]
Western Blot: Frataxin Antibody (3G9) [H00002395-M03] - Analysis of FXN expression in transfected 293T cell line by FXN monoclonal antibody (M03), clone 3G9. Lane 1: FXN transfected lysatE (23.1 KDa). Lane 2: Non-transfected lysate.![Western Blot: Frataxin Antibody (3G9) [H00002395-M03]](https://resources.bio-techne.com/images/products/Frataxin-Antibody-3G9-Western-Blot-H00002395-M03-img0001.jpg "Western Blot: Frataxin Antibody (3G9) [H00002395-M03]")
Western Blot: Frataxin Antibody (3G9) [H00002395-M03]
Western Blot: Frataxin Antibody (3G9) [H00002395-M03] - Western blot analysis of FXN over-expressed 293 cell line, cotransfected with FXN Validated Chimera RNAi or non-transfected control. Blot probed with H00002395-M03. GAPDH (36.1 kDa) used as loading control.Applications for Frataxin Antibody (3G9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi validaton and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Frataxin
Alternate Names
CyaY, EC 1.16.3.1, FA, FARRmitochondrial, frataxin, Friedreich ataxia, Friedreich ataxia protein, Fxn, X25MGC57199
Entrez Gene IDs
2395 (Human)
Gene Symbol
FXN
OMIM
229300 (Human)
UniProt
Additional Frataxin Products
Product Documents for Frataxin Antibody (3G9)
Product Specific Notices for Frataxin Antibody (3G9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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