Skip to main content

Frataxin Antibody (FXN/2124)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79802

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # FXN/2124

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human Frataxin (FXN) protein (around aa 57-210) (exact sequence is proprietary) (Uniprot: Q16595)

Localization

Cytoplasmic (Mitochondria)

Marker

Marker of Friedreich Ataxia

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79936)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Frataxin Antibody (FXN/2124)

Western Blot: Frataxin Antibody (FXN/2124) [NBP2-79802]

Western Blot: Frataxin Antibody (FXN/2124) [NBP2-79802]

Western Blot: Frataxin Antibody (FXN/2124) [NBP2-79802] - Western Blot Analysis of Human Panc-2 and HepG2 cell lysate using Frataxin Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802]

Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802]

Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802]

Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802]

Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Antibody (FXN/2124).

Applications for Frataxin Antibody (FXN/2124)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Frataxin

Frataxin encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Alternate Names

CyaY, EC 1.16.3.1, FA, FARRmitochondrial, frataxin, Friedreich ataxia, Friedreich ataxia protein, Fxn, X25MGC57199

Gene Symbol

FXN

UniProt

Additional Frataxin Products

Product Documents for Frataxin Antibody (FXN/2124)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Frataxin Antibody (FXN/2124)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...