Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 162531

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Frizzled-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln72-His248 (Met122Ile)
Accession # O70421

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Frizzled-1 Antibody (162531) [DyLight 550]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Frizzled-1

FZD1 is a widely expressed Frizzled Receptor that mediates Wnt signaling. FZD1 expression has been reported in heart, kidney, lung, ovary, pancreas, placenta, and prostate. ESTs have been isolated from brain, embryo, eye, heart, heart/melanocyte/uterus, kidney, liver/spleen, lung, prostate, skin, and uterus libraries.

Alternate Names

Frizzled1, FZD1

Gene Symbol

FZD1

Additional Frizzled-1 Products

Product Documents for Frizzled-1 Antibody (162531) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Frizzled-1 Antibody (162531) [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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