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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 169310

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086

Specificity

Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Frizzled-3 Antibody (169310) [DyLight 550]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Frizzled-3

Frizzled-3 is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

Alternate Names

Frizzled3, FZD3

Gene Symbol

FZD3

Additional Frizzled-3 Products

Product Documents for Frizzled-3 Antibody (169310) [DyLight 550]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Frizzled-3 Antibody (169310) [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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