Frizzled-3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14325
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Avian (100%), Bat (100%), Bovine (100%), Canine (100%), Chicken (100%), Equine (100%), Hamster (100%), Monkey (100%), Mouse (100%), Opossum (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic 18 amino acid peptide from N-Terminus extracellular domain of human Frizzled-3.
Epitope
N-Terminus
Reactivity Notes
Expected reactivity based on percent sequence identity: Xenopus (89%), Zebrafish (83%)
Specificity
Human Frizzled-3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for Frizzled-3 Antibody
![Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]](https://resources.bio-techne.com/images/products/Frizzled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-14325-img0001.jpg "Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]")
Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]
Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]](https://resources.bio-techne.com/images/products/Frizzled-3 Antibody-Immunohistochemistry-Paraffin-NBP3-14325-img0002.jpg "Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]")
Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325]
Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.Applications for Frizzled-3 Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 3-5 ug/ml..
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: Frizzled-3
Alternate Names
Frizzled3, FZD3
Gene Symbol
FZD3
Additional Frizzled-3 Products
Product Documents for Frizzled-3 Antibody
Product Specific Notices for Frizzled-3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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