Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Chimpanzee (100%), Monkey (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Frizzled-6 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids in the central domain of human Frizzled-6. The immunogen is located within 50-550 amino acids of Frizzled-6.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Frizzled-6 Antibody - BSA Free

Western Blot: Frizzled-6 AntibodyBSA Free [NBP3-18335]

Western Blot: Frizzled-6 AntibodyBSA Free [NBP3-18335]

Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
Frizzled-6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] -

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Mouse Lung.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Frizzled-6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] -

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Applications for Frizzled-6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Frizzled-6

FZD6 is a Frizzled Receptor for Wnt signaling proteins involved in patterning during development. Contrary to other Frizzled proteins, FZD6 does not contain a C-terminal PDZ domain-binding motif. FZD6 is widely expressed. The Drosophila homolog activates Dishevelled. FZD6 expression has been reported in brain, colon, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, testis, and thymus. Various tumor cell lines have also been shown to express FZD6. ESTs have been isolated from blood, brain, breast, connective tissue, liver, liver/spleen, lung, placenta, testis, thyroid, and vessel libraries. In addition, several ESTs have been isolated from tumor libraries.

Alternate Names

Frizzled6, FZD6

Gene Symbol

FZD6

Additional Frizzled-6 Products

Product Documents for Frizzled-6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Frizzled-6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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