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Frizzled-7 Antibody (4D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00008324-M03

Novus Biologicals, part of Bio-Techne
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H00008324-M03-0.05mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

FZD7 (NP_003498.1, 155 a.a. ~ 253 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GAGEICVGQNTSDGSGGPGGGPTAYPTAPYLPDLPFTALPPGASDGRGRPAFPFSCPRQLKVPPYLGYRFLGERDCGAPCEPGRANGLMYFKEEERRFA

Specificity

frizzled homolog 7 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Frizzled-7 Antibody (4D9)

Western Blot: Frizzled-7 Antibody (4D9) [H00008324-M03]

Western Blot: Frizzled-7 Antibody (4D9) [H00008324-M03]

Western Blot: Frizzled-7 Antibody (4D9) [H00008324-M03] - Analysis of FZD7 expression in K-562 (Cat # L009V1).
Immunohistochemistry-Paraffin: Frizzled-7 Antibody (4D9) [H00008324-M03]

Immunohistochemistry-Paraffin: Frizzled-7 Antibody (4D9) [H00008324-M03]

Immunohistochemistry-Paraffin: Frizzled-7 Antibody (4D9) [H00008324-M03] - Analysis of monoclonal antibody to FZD7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
ELISA: Frizzled-7 Antibody (4D9) [H00008324-M03]

ELISA: Frizzled-7 Antibody (4D9) [H00008324-M03]

ELISA: Frizzled-7 Antibody (4D9) [H00008324-M03] - Detection limit for recombinant GST tagged FZD7 is 0.3 ng/ml as a capture antibody.

Applications for Frizzled-7 Antibody (4D9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Frizzled-7

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq]

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human)

Gene Symbol

FZD7

UniProt

Additional Frizzled-7 Products

Product Documents for Frizzled-7 Antibody (4D9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Frizzled-7 Antibody (4D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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