Frizzled-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2439

Novus Biologicals, part of Bio-Techne
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NB100-2439
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Frizzled-8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SYPKQMPLSQV corresponding to C-Terminus according to NP_114072.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22817897).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Frizzled-8 Antibody

Western Blot: Frizzled-8 Antibody [NB100-2439]

Western Blot: Frizzled-8 Antibody [NB100-2439]

Western Blot: Frizzled-8 Antibody [NB100-2439] - Staining of human pancreas lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NB100-2439]

Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NB100-2439]

Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NB100-2439] - Staining of paraffin embedded Human Pancreas. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-detection.

Applications for Frizzled-8 Antibody

Application
Recommended Usage

Immunohistochemistry

3 ug/mL

Immunohistochemistry-Paraffin

3 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 75 kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3 kDa band according to NP_114072.1). IHC-P: Human pancreas show granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Frizzled-8

Gene ID(s): 8325 (human)

Alternate Names

Frizzled8, FZD8

Entrez Gene IDs

8325 (Human); 14370 (Mouse)

Gene Symbol

FZD8

UniProt

Q9H461

Additional Frizzled-8 Products

Product Documents for Frizzled-8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Frizzled-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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