FRS2 Antibody (HL1774) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25470

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25470
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1774

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FRS2 Antibodies »

Product Summary for FRS2 Antibody (HL1774) - Azide and BSA Free

Immunogen

Recombinant fragment of human FRS2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FRS2 Antibody (HL1774) - Azide and BSA Free

FRS2 Antibody (HL1774) - Azide and BSA Free

Western Blot: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] -

Western Blot: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody [HL1774] (NBP3-25470) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FRS2 Antibody (HL1774) - Azide and BSA Free

Western Blot: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] -

Western Blot: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody [HL1774] (NBP3-25470) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FRS2 Antibody (HL1774) - Azide and BSA Free

Knockdown Validated: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] -

Knockdown Validated: FRS2 Antibody (HL1774) - Azide and BSA Free [NBP3-25470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody [HL1774] (NBP3-25470) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for FRS2 Antibody (HL1774) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FRS2

FGFs (Fibroblast Growth Factors) bind to FGF receptors (FGFRs) monovalently, and FGF receptor dimerization and activation is mediated by multivalent interactions between heparin sulfate proteoglycans and FGF. Upon activation, receptor tyrosine kinases undergo rapid autophosphorylation on numerous tyrosine residues. Autophosphorylation sites located within the catalytic domain are crucial for stimulation of kinase activity, while autophosphorylation sites located in other regions are usually involved in the recruitment of cellular target proteins. Fibroblast growth factor or nerve growth factor (NGF) stimulation leads to tyrosine phosphorylation of the FGF Receptor Substrate 2 (FRS2) docking proteins. FRS2 is also designated SNT (Suc-1 associated neurotrophic factor target protein). Two structurally similar forms of FRS2 are: FRS2a (SNT-1) and FRS-2b (SNT-2, FRS3). FRS functions as a lipid-anchored docking protein that is tyrosine phosphorylated and recruited to FGFR upon FGF stimulation. FRS2 plays an important role in linking FGF and NGF with the Ras/MAPK signaling pathway, thus relaying information from the cell surface to the nucleus. FRS2 contains both a consensus myristylation sequence, involved in its recruitment to the cell membrane and a putative phosphotyrosine binding (PTB)domain in its amino-terminus. The interaction of FRS2 with FGFR1 occurs via the PTB domain of FRS2 binding to a 12-residue segment in the juxtamembrane region of FGFR1. FRS2a contains four binding sites for the adaptor protein Grb2 and two binding sites for the protein tyrosine phosphatase Shp2. FGF stimulation leads to phosphorylation of Shp2 on a tyrosine residue forming a complex with an additional molecule of Grb2. Grb2/Sos complexes are thus recruited directly and indirectly via Shp2 upon tyrosine phosphorylation of FRS2a in response to growth factor stimulation. It has been shown that FRS2 proteins are tyrosine phos phorylated in response to activation of the RET receptor, a tyrosine kinase that functions as the signal transducing receptor for the GDNF.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Gene Symbol

FRS2

Additional FRS2 Products

Product Documents for FRS2 Antibody (HL1774) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FRS2 Antibody (HL1774) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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