FTO Antibody (4G9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26565
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4G9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human FTO [UniProt Q9C0B1]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FTO Antibody (4G9)
Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] -
Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] -
Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: FTO Antibody (4G9) [NBP3-26565] -
Western Blot: FTO Antibody (4G9) [NBP3-26565] - Positive Western Blot detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate.All lanes: FTO Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 59, 15, 7, 13 KDa
Observed band size: 59 KDa
Applications for FTO Antibody (4G9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: FTO
FTO antibodies are useful tools for lipid metabolism research and obesity studies.
Long Name
Fat Mass and Obesity Associated
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO
Gene Symbol
FTO
Additional FTO Products
Product Documents for FTO Antibody (4G9)
Product Specific Notices for FTO Antibody (4G9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...