Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all FUBP3 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 522 and 572 of human FUBP3 using the numbering given in entry NP_003925.1 (GeneID 8939).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FUBP3 Antibody - BSA Free

Immunoprecipitation: FUBP3 Antibody [NBP1-50045]

Immunoprecipitation: FUBP3 Antibody [NBP1-50045]

Immunoprecipitation: FUBP3 Antibody [NBP1-50045] - Immunoprecipitation: FUBP3 Antibody [NBP1-50045] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FBP3 antibody NBP1-50044 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FBP3 was also immunoprecipitated by rabbit anti-FBP3 antibody NBP1-50045, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Applications for FUBP3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FUBP3

Activation of FUBP3, the far-upstream element, is required for the proper expression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUBP3 elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxylterminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation.

Alternate Names

far upstream element (FUSE) binding protein 3, far upstream element-binding protein 3, FBP3, FLJ25229, FUSE-binding protein 3

Gene Symbol

FUBP3

Additional FUBP3 Products

Product Documents for FUBP3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FUBP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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