Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27142

Novus Biologicals, part of Bio-Techne
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NBP3-27142-0.025ml
NBP3-27142-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F8G5C7

Concentration

1 mg/ml

View all Fucosyltransferase 3/FUT3 Antibodies »

Product Summary for Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

Immunogen

Purified recombinant fragment of human Fucosyltransferase 3/FUT3 (AA: 199-361) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

ELISA: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] -

ELISA: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow Cytometry: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] -

Flow Cytometry: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] - Analysis of HL-60 cells using Fucosyltransferase 3/FUT3 mouse mAb (green) and negative control (red).

Western Blot: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] -

Western Blot: Fucosyltransferase 3/FUT3 Antibody (1F8G5C7) [NBP3-27142] - Analysis using Fucosyltransferase 3/FUT3 mAb against human Fucosyltransferase 3/FUT3 (AA: 199-361) recombinant protein. (Expected MW is 45.5 kDa)
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Applications for Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fucosyltransferase 3/FUT3

Because N-, O-glycans and glycolipids are frequently fucosylated at terminal sites, fucose is often found to be essential for sugar epitope and lectin ligand generation. Well-known fucose containing structures include Lewis structures and ABO blood group antigens. Lewis structures are key elements involved in leukocyte homing and extravasation process and thus are essential for lymphocyte maturation and natural defense functions. Fucose containing glycans also play essential roles in cell signaling and development. So far, more than 10 fucosyltransferases have been cloned from the human genome. FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis. FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are responsible for Lewis structure generation through their alpha 1-3 or alpha 1-4 fucosyltransferases activities. FUT3, also known as Lewis blood group fucosyltransferase, is unique by having both strong alpha 1-3 and alpha 1-4 fucosyltransferase activities. FUT3 has high homology with FUT5 and FUT6 due to gene duplication. FUT7 is exclusively responsible for biosynthesis of sialyl Lewis X epitope in leukocytes and high endothelial venule cells. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans. Predicted as type II transmembrane proteins and Golgi enzymes, some of the fucosyltransferases can also be found in plasma. R&D Systems rhFUTs correspond to the luminal domains.

Alternate Names

CD174, FT3B, FucT-III, FUT3, Les, Lewis FT

Gene Symbol

FUT3

Additional Fucosyltransferase 3/FUT3 Products

Product Documents for Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fucosyltransferase 3/FUT3 Antibody (1F8G5C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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