Fumarase Antibody (OTI1F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47754

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F10

Concentration

1 mg/ml

View all Fumarase Antibodies »

Product Summary for Fumarase Antibody (OTI1F10)

Immunogen

Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens fumarate hydratase (FH), nuclear gene encoding mitochondrial protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (OTI1F10)

Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.
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Applications for Fumarase Antibody (OTI1F10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fumarase

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form,differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion,where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Entrez Gene IDs

2271 (Human); 24368 (Rat)

Gene Symbol

FH

UniProt

P07954 (Human)

Additional Fumarase Products

Product Documents for Fumarase Antibody (OTI1F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fumarase Antibody (OTI1F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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