Fumarase Antibody (S08-3F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15029

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15029-100ul
NBP3-15029-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-3F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fumarase Antibodies »

Product Summary for Fumarase Antibody (S08-3F2)

Immunogen

A synthetic peptide of human Fumarase (Uniprot#: P07954)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (S08-3F2)

Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029]

Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029]

Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029]

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029]

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029] - Immunofluorescence of Fumarase (green) in hela using Fumarase Rabbit mAb at dilution 1/50, and DAPI (blue)

Applications for Fumarase Antibody (S08-3F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Western Blot

1/1000-1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fumarase

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Gene Symbol

FH

Additional Fumarase Products

Product Documents for Fumarase Antibody (S08-3F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Fumarase Antibody (S08-3F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.