FUS Antibody (CL0190)

Knockout Validated: FUS Antibody (CL0190) [NBP2-52874]

Knockout Validated: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validated: FUS Antibody (CL0190) [NBP2-52874]

Knockout Validated: FUS Antibody (CL0190) [NBP2-52874] - Western blot using lysates of HeLa parental cell line (WT) and FUS knockout HeLa cell line (KO), collected in RIPA Buffer. Lysates were prepared using 30 ug of protein and the nitrocellulose membrane was probed with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:5000 O/N at 4C, followed by peroxidase-conjugated Anti-Mouse Secondary Antibody and ECL detection. A specific band at ~70 kDa was detected in the FUS WT cell line but is not observed in the FUS KO cell line. The Ponceau stained transfer of the blot is shown to confirm equal protein loading. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874]

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining in human cerebral cortex and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.