FUS Antibody (HL2454) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25471

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25471
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2454

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FUS Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human FUS.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FUS Antibody (HL2454) - Azide and BSA Free

FUS Antibody (HL2454) - Azide and BSA Free

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FUS Antibody (HL2454) - Azide and BSA Free

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. FUS stained by FUS antibody [HL2454] (NBP3-25471) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FUS Antibody (HL2454) - Azide and BSA Free

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for FUS Antibody (HL2454) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUS

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Gene Symbol

FUS

Additional FUS Products

Product Documents for FUS Antibody (HL2454) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FUS Antibody (HL2454) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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