Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FUS Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FUS Antibody

Knockout Validated: FUS Antibody [NB100-562]

Knockout Validated: FUS Antibody [NB100-562]

Knockout Validated: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Western Blot: FUS Antibody [NB100-562]

Western Blot: FUS Antibody [NB100-562]

Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: FUS Antibody [NB100-562]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-562]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
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Applications for FUS Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:10000

Immunohistochemistry-Paraffin

1:1000-1:10000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000-1:50000
Application Notes
Prepare working dilution immediately before use. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-562 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FUS

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Entrez Gene IDs

2521 (Human)

Gene Symbol

FUS

UniProt

P35637 (Human)

Additional FUS Products

Product Documents for FUS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FUS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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