FXR/NR1H4 Antibody (1B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00009971-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FXR/NR1H4 Antibodies »

Product Specifications

Immunogen

NR1H4 (NP_005114, 363 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ

Specificity

nuclear receptor subfamily 1, group H, member 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FXR/NR1H4 Antibody (1B10)

Western Blot: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

Western Blot: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

Western Blot: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - NR1H4 monoclonal antibody (M02), clone 1B10 Analysis of NR1H4 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

Immunocytochemistry/ Immunofluorescence: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - Analysis of monoclonal antibody to NR1H4 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

ELISA: FXR/NR1H4 Antibody (1B10) [H00009971-M02]

ELISA: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - Detection limit for recombinant GST tagged NR1H4 is approximately 0.03ng/ml as a capture antibody.

Applications for FXR/NR1H4 Antibody (1B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FXR/NR1H4

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Entrez Gene IDs

9971 (Human)

Gene Symbol

NR1H4

OMIM

603826 (Human)

UniProt

NP_005114 (Human)

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody (1B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FXR/NR1H4 Antibody (1B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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