Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FXR/NR1H4 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 21-297 of human NR1H4 (NP_005114) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free

Western Blot: FXR/NR1H4 Antibody (OTI4F12)Azide and BSA Free [NBP2-71526]

Western Blot: FXR/NR1H4 Antibody (OTI4F12)Azide and BSA Free [NBP2-71526]

Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4
Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526]

Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526]

Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.

Applications for FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXR/NR1H4

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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