Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FXYD3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FXYD3(NP_005962) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

7.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FXYD3 Antibody (OTI4E3) - Azide and BSA Free

Western Blot: FXYD3 Antibody (OTI4E3)Azide and BSA Free [NBP2-71219]

Western Blot: FXYD3 Antibody (OTI4E3)Azide and BSA Free [NBP2-71219]

Western Blot: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXYD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXYD3.
Immunocytochemistry/ Immunofluorescence: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219]

Immunocytochemistry/ Immunofluorescence: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219]

Immunocytochemistry/Immunofluorescence: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FXYD3.
Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219]

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219]

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Human liver tissue using anti-FXYD3 mouse monoclonal antibody.
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Applications for FXYD3 Antibody (OTI4E3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXYD3

FXYD3, also known as FXYD domain-containing ion transport regulator 3, consists of 3 isoforms, a 87 amino acid isoform that is 9 kDa, a 113 amino acid isoform that is 12 kDa, and a 144 amino acid that is 15 kDa; is membrane located, functions as an inducer of a hyperpolarization-activated chloride current when expressed in Xenopus oocytes, and may be a modulator capable of activating endogenous oocyte channels. Current research is being performed on several diseases and disorders including adenocarcinoma, pancreatic, ductal adenocarcinoma, intrahepatic cholangiocarcinoma, colorectal cancer, cholangiocarcinoma prostate carcinoma, melanoma, pancreatic cancer, pancreatitis, prostate cancer, breast cancer, and prostatitis. The FXYD3 protein has also shown an interaction with SERBP1, FBXL12, NR4A1, MAPK6 and NUDT3, in the regulation of catalytic activity, ion transport, and chloride transport pathways.

Alternate Names

Chloride conductance inducer protein Mat-8, FXYD domain containing ion transport regulator 3, FXYD domain-containing ion transport regulator 3, Mammary tumor 8 kDa protein, MAT8MAT-8, Phospholemman-like, phospholemman-like protein, PLMLMGC111076

Gene Symbol

FXYD3

Additional FXYD3 Products

Product Documents for FXYD3 Antibody (OTI4E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FXYD3 Antibody (OTI4E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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