FXYD5/Dysadherin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1003

Novus Biologicals, part of Bio-Techne
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NB100-1003
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FXYD5/Dysadherin Antibodies »

Product Specifications

Immunogen

Peptide with sequence GKCRQLSRLCRNHCR corresponding to C-Terminus according to NP_054883.3, NP_659003.1, NP_001158077.1.

Specificity

NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FXYD5/Dysadherin Antibody

Western Blot: FXYD5/Dysadherin Antibody [NB100-1003]

Western Blot: FXYD5/Dysadherin Antibody [NB100-1003]

Western Blot: FXYD5/Dysadherin Antibody [NB100-1003] - (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for FXYD5/Dysadherin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human spleen lysate and an approx. 26 kDa band in human lung lysate after 1 ug/ml antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylatedscientific literature (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa band according to NP_054883 and NP_659003.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXYD5/Dysadherin

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane.

Long Name

FXYD Domain-containing Ion Transport Regulator 5

Alternate Names

DYSAD, Dysadherin, EF-8, IWU1, KCT1, OIT2, PRO6241, RIC

Entrez Gene IDs

53827 (Human)

Gene Symbol

FXYD5

UniProt

NP_054883. (Human)

Additional FXYD5/Dysadherin Products

Product Documents for FXYD5/Dysadherin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FXYD5/Dysadherin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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