FYCO1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47266
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1428 and 1478 of human FYVE and Coiled-Coil Domain Containing 1 using the numbering given in entry NP_078789.2 (GeneID 79443).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 22619176).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FYCO1 Antibody
![Western Blot: FYCO1 Antibody [NBP1-47266]](https://resources.bio-techne.com/images/products/FYCO1-Antibody-Western-Blot-NBP1-47266-img0001.jpg "Western Blot: FYCO1 Antibody [NBP1-47266]")
Western Blot: FYCO1 Antibody [NBP1-47266]
Western Blot: FYCO1 Antibody [NBP1-47266] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FYCO1 antibody NBP1-47266 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. FYCO1 was also immunoprecipitated by rabbit anti-FYCO1 antibody NBP1-47265, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for FYCO1 Antibody
Application
Recommended Usage
Immunoprecipitation
5-15 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FYCO1
Alternate Names
DKFZp779K1152, FLJ13335, FYVE and coiled-coil domain containing 1, FYVE and coiled-coil domain-containing protein 1, MGC126517, RUFY3, RUN and FYVE domain containing 3, ZFYVE7MGC126519, Zinc finger FYVE domain-containing protein 7
Entrez Gene IDs
79443 (Human)
Gene Symbol
FYCO1
UniProt
Additional FYCO1 Products
Product Documents for FYCO1 Antibody
Product Specific Notices for FYCO1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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