FZR1/CDH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15840

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-15840
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (97%), Chicken (94%), Porcine (97%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FZR1/CDH1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FZR1/CDH1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Localization

Isoform 2: Nucleus; Isoform 3: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FZR1/CDH1 Antibody

Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - Cdh1 decreases upon Abeta treatment in neurons. Representative Western blot image of cdh1 after different time points in control conditions or with Abeta treatment. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep31158), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840]

Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840]

Immunocytochemistry/Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse brain. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 3 more images

Applications for FZR1/CDH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FZR1/CDH1

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Alternate Names

CDC20C, CDH1, Cdh1/Hct1 homolog, CDH1CDC20-like 1b, fizzy/cell division cycle 20 related 1 (Drosophila), Fzr, FZR2, FZRCDC20-like protein 1, HCDH1, HCDHFYR, KIAA1242fizzy-related protein homolog

Gene Symbol

FZR1

UniProt

Q9UM11 (Human)

Additional FZR1/CDH1 Products

Product Documents for FZR1/CDH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FZR1/CDH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.