G3BP1 Antibody (5A4T0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16302
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A4T0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human G3BP1 (Q13283). EVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for G3BP1 Antibody (5A4T0)
Western Blot: G3BP1 Antibody (5A4T0) [NBP3-16302]
Western Blot: G3BP1 Antibody (5A4T0) [NBP3-16302] - Western blot analysis of extracts of various cell lines, using G3BP11 Rabbit mAb (NBP3-16302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]
Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Immunofluorescence analysis of NIH-3T3 cells using G3BP11 Rabbit mAb (NBP3-16302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]
Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Immunofluorescence analysis of HeLa cells using G3BP11 Rabbit mAb (NBP3-16302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for G3BP1 Antibody (5A4T0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: G3BP1
Alternate Names
ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP
Gene Symbol
G3BP1
Additional G3BP1 Products
Product Documents for G3BP1 Antibody (5A4T0)
Product Specific Notices for G3BP1 Antibody (5A4T0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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