G3BP1 Antibody (S09-5B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15030

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15030-100ul
NBP3-15030-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-5B9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all G3BP1 Antibodies »

Product Summary for G3BP1 Antibody (S09-5B9)

Immunogen

A synthetic peptide of human G3BP1 (Uniprot#: Q13283)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G3BP1 Antibody (S09-5B9)

Western Blot: G3BP1 Antibody (S09-5B9) [NBP3-15030]

Western Blot: G3BP1 Antibody (S09-5B9) [NBP3-15030]

Western Blot: G3BP1 Antibody (S09-5B9) [NBP3-15030] - Western blot detection of G3BP1 in K562, C6, Hela cell lysates using G3BP1 Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 68kDa.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (S09-5B9) [NBP3-15030]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (S09-5B9) [NBP3-15030]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (S09-5B9) [NBP3-15030] - Immunofluorescence of G3BP1 (green) in Hela cells using G3BP1 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for G3BP1 Antibody (S09-5B9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50-1/200

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G3BP1

G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Gene Symbol

G3BP1

Additional G3BP1 Products

Product Documents for G3BP1 Antibody (S09-5B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for G3BP1 Antibody (S09-5B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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