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G9a/EHMT2 Antibody (SR1220)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22401

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22401-100ul
NBP3-22401-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1220

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for G9a/EHMT2 Antibody (SR1220)

Immunogen

A synthesized peptide derived from G9a/EHMT2 (Uniprot #: Q96KQ7)

Specificity

Detects endogenous levels of total G9a/EHMT2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G9a/EHMT2 Antibody (SR1220)

Western Blot: G9a/EHMT2 Antibody (SR1220) [NBP3-22401] -

Western Blot: G9a/EHMT2 Antibody (SR1220) [NBP3-22401] - Western blot analysis using NBP3-22401 in 293 cell lysate.

Applications for G9a/EHMT2 Antibody (SR1220)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Gene Symbol

EHMT2

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody (SR1220)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for G9a/EHMT2 Antibody (SR1220)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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