G9a/EHMT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55881

Novus Biologicals, part of Bio-Techne
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NBP2-55881
NBP2-55881-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all G9a/EHMT2 Antibodies »

Product Summary for G9a/EHMT2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSS

Reactivity Notes

Mouse 85%, Rat 87%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G9a/EHMT2 Antibody

Western Blot: G9a/EHMT2 Antibody [NBP2-55881]

Western Blot: G9a/EHMT2 Antibody [NBP2-55881]

Western Blot: G9a/EHMT2 Antibody [NBP2-55881] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881]

Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881]

Immunocytochemistry/Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear speckles. Antibody staining is shown in green.

Applications for G9a/EHMT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Gene Symbol

EHMT2

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for G9a/EHMT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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