GABA-A R alpha 6 C-Terminus Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS028

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GABA-A R alpha 6 Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytosolic loop of the rat GABAA R alpha6 subunit, C-Terminus

Specificity

Human, mouse, rat, and bovine ~57 kDa GABAA R alpha6 subunit, C-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABA-A R alpha 6 C-Terminus Antibody

Detection of GABAAR a6 C-Terminus antibody by Western Blot.

Detection of GABAAR alpha6 C-Terminus by Western Blot.

Western blot of forebrain lysates from wild type and alpha6 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAAR, alpha6 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~57 kDa alpha6 subunit of the GABAAR in the wild type (control) but not in the alpha6 knockout animals.

Applications for GABA-A R alpha 6 C-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Mouse brain (forebrain) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 6

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha6 isoform is a 57 kDa, 434 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The expression of the subunit is strongly associated with cerebellar granule cells. The alpha6 subunit has also been linked to alcohol sensitivity, possible due to a point mutation substitution of arginine for a glutamine at amino acid position 81 in the extracellular N-terminus.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Korpi, E.R. et al. (1993) Nature 361:356.
  4. Luddens, H. et al. (1990) Nature 346:648.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-6 Polypeptide

Alternate Names

GABAAR alpha 6, GABAARa6, GABRA6

Entrez Gene IDs

2559 (Human); 14399 (Mouse); 29708 (Rat)

Gene Symbol

GABRA6

Additional GABA-A R alpha 6 Products

Product Documents for GABA-A R alpha 6 C-Terminus Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GABA-A R alpha 6 C-Terminus Antibody

For research use only

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