Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N87/25

Concentration

1 mg/ml

View all GABA-A R beta 3 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane

Specificity

Detects approx 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R beta 3 Antibody (N87/25)

Western Blot: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Western Blot: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Western Blot: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-A R beta 3 protein using Mouse Anti-GABA-A R beta 3 Monoclonal Antibody, Clone N87/25 (NBP1-47613). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA-A R beta 3 Monoclonal Antibody (NBP1-47613) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Immunocytochemistry/ Immunofluorescence: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Immunocytochemistry/Immunofluorescence: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25 (NBP1-47613). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody (NBP1-47613) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.
Immunohistochemistry: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Immunohistochemistry: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613]

Immunohistochemistry: GABA-A R beta 3 Antibody (N87/25) [NBP1-47613] - Immunohistochemistry analysis using Mouse Anti-GABA-A R beta 3 Monoclonal Antibody, Clone N87/25 (NBP1-47613). Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA-A R beta 3 Monoclonal Antibody (NBP1-47613) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.

Applications for GABA-A R beta 3 Antibody (N87/25)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of GABA-A Receptor Beta3 Antibody was sufficient for detection of Beta3 GABA receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA-A R beta 3

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide

Alternate Names

GABAAR beta 3, GABAARb3, GABRB3

Entrez Gene IDs

2562 (Human); 14402 (Mouse); 24922 (Rat)

Gene Symbol

GABRB3

UniProt

P63080 (Mouse)

Additional GABA-A R beta 3 Products

Product Documents for GABA-A R beta 3 Antibody (N87/25)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GABA-A R beta 3 Antibody (N87/25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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