GABA-A R gamma 2 [p Ser327] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28661

Novus Biologicals, part of Bio-Techne
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NBP1-28661
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-A R gamma 2 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH. Accession # P18508

Modification

p Ser327

Specificity

Specific for endogenous levels of the ~45 kDa GABAA receptor gamma 2 subunit phosphorylated at Ser327. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R gamma 2 [p Ser327] Antibody

Western Blot: GABA-A R gamma 2 [p Ser327] Antibody [NBP1-28661]

Western Blot: GABA-A R gamma 2 [p Ser327] Antibody [NBP1-28661]

Western Blot: GABA-A R gamma 2 [p Ser327] Antibody [NBP1-28661] - Western blot of rat cortex showing specific immunolabeling of the ~45k GABA-A R gamma 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Applications for GABA-A R gamma 2 [p Ser327] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R gamma 2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKC-E regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)

Long Name

gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

Alternate Names

GABAAR gamma 2, GABAARg2, GABRG2

Gene Symbol

GABRG2

UniProt

P18508 (Rat)

Additional GABA-A R gamma 2 Products

Product Documents for GABA-A R gamma 2 [p Ser327] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-A R gamma 2 [p Ser327] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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