GABA-A R gamma 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS072

R&D Systems, part of Bio-Techne
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PPS072
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chicken, Primate, Zebrafish

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

View all GABA-A R gamma 2 Antibodies »

Product Specifications

Immunogen

GABA-A R gamma 2

Specificity

Bovine, human, mouse, and rat ~44-47 kDa gamma2-subunit of the GABAA Receptor in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for GABA-A R gamma 2 Antibody

Detection of GABAAR ?2 antibody by Western Blot.

Detection of GABAAR gamma2 by Western Blot.

Western blot of rat brain lysate showing specific immunolabeling of the approximately 44-47 kDa gamma 2 subunit of the GABAAR.

Applications for GABA-A R gamma 2 Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50 μL of unpurified rabbit serum.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R gamma 2

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha,  beta,  gamma,  delta,  epsilon,  theta, and π. Three subunits, alpha, beta, and  gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of an alpha, beta and gamma subunit. The rat gamma2 isoform is a 48 kDa, 436 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 30-233). The gamma2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha1 beta2 gamma2). GABA binds at alpha-beta interfaces, while benzodiazepine binds to alpha-gamma interfaces. There are two splice forms, the longest that contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa 376-383. PKC phosphorylates the long form at S381, while both the short and long forms are phosphorylated at S365. Phosphorylation blocks receptor activity. gamma2 subunits are also palmitoylated at multiple sites on cysteines that lie between aa 415-461, facilitating membrane trafficking.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
  4. Krishek, B.J. et al. (1994) Neuron 12:1081.
  5. Keller, C.A. et al. (2004) J. Neurosci. 24:5881.
  6. Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.

Long Name

gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

Alternate Names

GABAAR gamma 2, GABAARg2, GABRG2

Entrez Gene IDs

2566 (Human); 14406 (Mouse); 29709 (Rat); 282240 (Bovine)

Gene Symbol

GABRG2

Additional GABA-A R gamma 2 Products

Product Documents for GABA-A R gamma 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-A R gamma 2 Antibody

For research use only

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