GABA-AR alpha 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61096

Novus Biologicals, part of Bio-Techne
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NB100-61096
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (%), Canine (%), Human (%), Primate (%), Zebrafish (%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA A R alpha 3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the N-terminal region of the alpha 3 subunit. Accession # P20236

Specificity

Specific for endogenous levels of the ~51 kDa alpha3-subunit of the GABAA receptor. Immunolabeling is absent in alpha3-subunit knockout animals.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 3 Antibody

Western Blot: GABA-AR alpha 3 Antibody [NB100-61096]

Western Blot: GABA-AR alpha 3 Antibody [NB100-61096]

Western Blot: GABA A R alpha 3 Antibody [NB100-61096] - Rat brain lysates from wild type (Control) and a3-knockout (a3-K/O) animals showing specific immunolabeling of the ~51k a3-subunit of the GABA A R. The labeling was absent from a lysate prepared from a3-knockout animals.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NB100-61096]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NB100-61096]

Immunohistochemistry: GABA A R alpha 3 Antibody [NB100-61096] - Immunostaining of rat amygdala showing labelling of GABA A R alpha 3

Applications for GABA-AR alpha 3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 19959723)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA A R alpha 3

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003).

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-3 Polypeptide

Alternate Names

GABAARa3, GABRA3

Entrez Gene IDs

2556 (Human); 14396 (Mouse); 24947 (Rat)

Gene Symbol

GABRA3

UniProt

P20236 (Rat)

Additional GABA A R alpha 3 Products

Product Documents for GABA-AR alpha 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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