GABA-AR alpha 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87499

Novus Biologicals, part of Bio-Techne
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NBP1-87499
NBP1-87499-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GABA A R alpha 3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSHCYMTSLGILFLINILPGTTGQGESRRQEPGDFVKQDIGGLSPKHAPDIPDDSTDNITIFTRILDRLLDGYDNRLRPGLGDAVTEVKTDIYVTSFGPVSDTDMEYTIDVFFRQTWHDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 3 Antibody

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.
View 6 more images

Applications for GABA-AR alpha 3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA A R alpha 3

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P ltl et al., 2003).

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-3 Polypeptide

Alternate Names

GABAARa3, GABRA3

Entrez Gene IDs

2556 (Human)

Gene Symbol

GABRA3

UniProt

P34903 (Human)

Additional GABA A R alpha 3 Products

Product Documents for GABA-AR alpha 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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