Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S415-24

Concentration

1 mg/ml

View all GABA-A R alpha 5 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.

Localization

Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane

Specificity

Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 5 Antibody (S415-24)

Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody, Clone S415-24 (NBP2-59700). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody (NBP2-59700) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-AR alpha 5 Alpha 5 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite.

Applications for GABA-AR alpha 5 Antibody (S415-24)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
A 1:100 dilution of this antibody was sufficient for detection of GABA-A R, Alpha5 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 5

The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide

Alternate Names

GABAAR alpha 5, GABAARa5, GABRA5

Gene Symbol

GABRA5

Additional GABA-A R alpha 5 Products

Product Documents for GABA-AR alpha 5 Antibody (S415-24)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABA-AR alpha 5 Antibody (S415-24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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