GABA-B R1 Antibody (JE60-09)

Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359]

Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: SHG-44 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 108 kDa
Observed band size: 108 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GABA-B R1 Antibody (JE60-09) [NBP3-32359]

Immunohistochemistry: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.