Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S93A-49

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-B R1 Antibodies »

Product Summary for GABA-B R1 Antibody (S93A-49)

Immunogen

Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane

Specificity

Detects 115kDa. No cross-reactivity against GABA(B)R2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GABA-B R1 Antibody (S93A-49)

Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-B R1 protein using Mouse Anti-GABA-B R1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Primary Antibody: Mouse Anti-GABA-B R1 Monoclonal Antibody (NBP2-59336) at 1:1000.
Immunocytochemistry/ Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Immunocytochemistry/ Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (NBP2-59336) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Immunocytochemistry/ Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336]

Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.

Applications for GABA-B R1 Antibody (S93A-49)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GABA-B R1

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 1

Alternate Names

GABABR1, GABBR1, Gb1, GPRC3A

Gene Symbol

GABBR1

Additional GABA-B R1 Products

Product Documents for GABA-B R1 Antibody (S93A-49)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GABA-B R1 Antibody (S93A-49)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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