Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S81-2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-B R2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 861-912 of rat GABA(B)R2

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane

Specificity

Detects 105kDa. No cross-reactivity against GABA(B)R1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GABA-B R2 Antibody (S81-2)

Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.
Immunocytochemistry/ Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Immunocytochemistry/ Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Immunocytochemistry/ Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335]

Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.
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Applications for GABA-B R2 Antibody (S81-2)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GABA-B R2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Moss and colleagues (Couve, et al., 2002) recently demonstrated that the functional coupling of GABAB R1/GABAB R2 receptors to inwardly rectifying K+ channels rapidly desensitizes. This effect is alleviated after direct phosphorylation of a single serine residue (Ser892) in the cytoplasmic tail of GABAB R2 by cyclic AMP (cAMP)-dependent protein kinase (PKA). In addition to it postsynaptic effects GABAB receptors localized to the presynaptic region have been reported to restrict the availability of synaptic vesicles for release (Sakaba and Neher, 2003).

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Gene Symbol

GABBR2

Additional GABA-B R2 Products

Product Documents for GABA-B R2 Antibody (S81-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GABA-B R2 Antibody (S81-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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