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GABA-B R2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16569

Novus Biologicals, part of Bio-Techne
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NBP2-16569

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABA-B R2. The exact sequence is proprietary.

Localization

Cell membrane; Multi-pass membrane protein; Cell junction; synapse; postsynaptic cell membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-B R2 Antibody

Western Blot: GABA-B R2 Antibody [NBP2-16569]

Western Blot: GABA-B R2 Antibody [NBP2-16569]

Western Blot: GABA-B R2 Antibody [NBP2-16569] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 2 antibody [N2C1], Internal diluted at 1:2000 and DDDDK tag antibody (NBP2-43574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: GABA-B R2 Antibody [NBP2-16569]

Immunohistochemistry-Frozen: GABA-B R2 Antibody [NBP2-16569]

Immunohistochemistry-Frozen: GABA-B R2 Antibody [NBP2-16569] - Frozen sectioned E13.5 Rat brain. Green: GABA B Receptor 2 protein stained by GABA B Receptor 2 antibody [N2C1], Internal (105986) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: GABA-B R2 Antibody [NBP2-16569]

Western Blot: GABA-B R2 Antibody [NBP2-16569]

Western Blot: GABA-B R2 Antibody [NBP2-16569] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Applications for GABA-B R2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GABA-B R2

B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM]

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Gene Symbol

GABBR2

UniProt

Additional GABA-B R2 Products

Product Documents for GABA-B R2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABA-B R2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.