GAD2/GAD65 Antibody (GAD2/1960)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79804

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # GAD2/1960

Concentration

0.2 mg/ml

View all GAD2/GAD65 Antibodies »

Product Summary for GAD2/GAD65 Antibody (GAD2/1960)

Immunogen

Recombinant human GAD2/GAD65 protein fragment (around aa 6-99) (exact sequence is proprietary) (Uniprot: Q05329)

Localization

Cytoplasmic

Marker

GABAergic Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79938)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GAD2/GAD65 Antibody (GAD2/1960)

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804] - Formalin-fixed, paraffin-embedded human Pancreas stained with GAD2/GAD65 Antibody (GAD2/1960).
Protein Array: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804]

Protein Array: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804]

Protein Array: GAD2/GAD65 Antibody (GAD2/1960) [NBP2-79804] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD2/GAD65 Antibody (GAD2/1960) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for GAD2/GAD65 Antibody (GAD2/1960)

Application
Recommended Usage

Immunohistochemistry

0.1-0.2 ug/ml

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

UniProt

Q05329

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (GAD2/1960)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD2/GAD65 Antibody (GAD2/1960)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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