GAD2/GAD65 Antibody (GAD2/8394R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20343
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # GAD2/8394R
Concentration
0.2 mg/ml
Product Summary for GAD2/GAD65 Antibody (GAD2/8394R)
Immunogen
Recombinant human GAD2/GAD65 protein fragment (around aa 1-200) (exact sequence is proprietary)
Localization
Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Human pancreas or brain (IHC).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for GAD2/GAD65 Antibody (GAD2/8394R)
GAD2/GAD65 Antibody (GAD2/8394R)
Formalin-fixed, paraffin-embedded human pancreas stained with GAD2/GAD65 antibody (GAD2/8394R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
GAD2/GAD65 Antibody (GAD2/8394R)
Formalin-fixed, paraffin-embedded human pancreas stained with GAD2/GAD65 antibody (GAD2/8394R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for GAD2/GAD65 Antibody (GAD2/8394R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GAD2/GAD65
Long Name
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)
Alternate Names
GAD65
Gene Symbol
GAD2
Additional GAD2/GAD65 Products
Product Documents for GAD2/GAD65 Antibody (GAD2/8394R)
Product Specific Notices for GAD2/GAD65 Antibody (GAD2/8394R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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