GADD34 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03703
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for GADD34 Antibody - Azide and BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human GADD34 (NP_055145.3). ASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GADD34 Antibody - Azide and BSA Free
![Western Blot: GADD34 AntibodyAzide and BSA Free [NBP3-03703]](https://resources.bio-techne.com/images/products/GADD34-Antibody-Western-Blot-NBP3-03703-img0001.jpg "Western Blot: GADD34 AntibodyAzide and BSA Free [NBP3-03703]")
Western Blot: GADD34 AntibodyAzide and BSA Free [NBP3-03703]
Western Blot: GADD34 Antibody [NBP3-03703] - Analysis of extracts of various cell lines, using GADD34 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]](https://resources.bio-techne.com/images/products/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03703-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]")
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]
Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NBP3-03703] - Immunofluorescence analysis of L929 cells using GADD34 Rabbit pAb (NBP3-03703) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]](https://resources.bio-techne.com/images/products/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03703-img0002.jpg "Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]")
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703]
Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NBP3-03703] - Immunofluorescence analysis of H9C2 cells using GADD34 Rabbit pAb (NBP3-03703) at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for GADD34 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GADD34
Long Name
Protein phosphatase 1 regulatory subunit 15A
Alternate Names
PPP1R15A
Gene Symbol
PPP1R15A
Additional GADD34 Products
Product Documents for GADD34 Antibody - Azide and BSA Free
Product Specific Notices for GADD34 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov