GADD45 alpha Antibody (3D12)
Novus Biologicals, part of Bio-Techne | Catalog # H00001647-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Simple Western, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3D12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER
Specificity
GADD45A - growth arrest and DNA-damage-inducible, alpha
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GADD45 alpha Antibody (3D12)
![Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01]](https://resources.bio-techne.com/images/products/GADD45-alpha-Antibody-3D12-Proximity-Ligation-Assay-H00001647-M01-img0001.jpg "Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01]")
Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01]
Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01] - Analysis of protein-protein interactions between MAP2K6 and GADD45A. HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for GADD45 alpha Antibody (3D12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.Use in Simple Western reported in scientific literature (PMID:29890265).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GADD45 alpha
Long Name
Growth Arrest and DNA Damage Gene alpha
Alternate Names
GADD45a
Entrez Gene IDs
1647 (Human)
Gene Symbol
GADD45A
OMIM
126335 (Human)
UniProt
Additional GADD45 alpha Products
Product Documents for GADD45 alpha Antibody (3D12)
Product Specific Notices for GADD45 alpha Antibody (3D12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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